Publications and Resources
Here you will find our publications and accompanying resources. For a full list of Nick’s work check out Google Scholar
Publications:
Cardiello JF, Araus AJ, Giatrellis S, Simon A, Leigh ND. Evaluation of genetic demultiplexing of single-cell sequencing data from model species. Life Science Alliance. 17 May 2023.
Leigh ND, Currie JD. Rebuilding limbs, one cell at a time. Developmental Dynamics. 16 February 2022.
Stegmayr J, Alsafadi HN, Langwinski W, Niroomand A, Lindstedt S, Leigh ND, Wagner DE. Isolation of high yield and quality RNA from human precision-cut lung slices for RNA-sequencing and computational integration with larger patient cohorts. AJP Lung and Cellular and Molecular Physiology. 28 Oct 2020.
Leigh ND, Sessa S., Dragalzew AC, Payzin-Dogru D, Sousa JF, Aggouras AN, Johnson K, Dunlap GS, Haas BJ, Levin M, Schneider I, Whited JL. von Willebrand factor D and EGF domains is an evolutionarily conserved and required feature of blastemas capable of multitissue appendage regeneration. Evolution & Development. 2020; 1– 15.
Leigh ND, Dunlap GS, Johnson K, Mariano R, Oshiro R, Wong AY, Bryant DM, Miller BM, Ratner A, Chen A, Ye WW, Haas BJ, Whited JL. Transcriptomic landscape of the blastema niche in regenerating adult axolotl limbs at single-cell resolution. Nature Communications 2018 9; 5153.
Pre-prints:
Cardiello JF, Araus AJ, Giatrellis S, Simon A, Leigh ND. Accurate genotype-based demultiplexing of single cell RNA sequencing samples from non-human animals. bioRxiv Septermber 23, 2022.
Stegmayr J, Alsafadi HN, Langwinski W, Niroomand A, Lindstedt S, Leigh ND, Wagner DE. Isolation of high yield and quality RNA from human precision-cut lung slices for RNA-sequencing and computational integration with larger patient cohorts. bioRxiv August 22, 2020.
Leigh ND, Sessa S., Dragalzew AC, Payzin-Dogru D, Sousa JF, Aggouras AN, Johnson K, Dunlap GS, Haas BJ, Levin M, Schneider I, Whited JL. von Willebrand factor D and EGF domains is an evolutionarily conserved and required feature of blastemas capable of multitissue appendage regeneration. bioRxiv November 15, 2019.
Resources:
Lab manual template generated in collaboration with eLife Ambassadors, this a fantastic resource to start a lab manual is forkable and freely available!
Guidelines Toward Inclusive Practices in Academics by eLife Community Ambassadors. Useful checklist-style guidelines to help scientists, mentors, funders, and journals to mitigate biases.
Check out regenerating axolotl limb single-cell RNAseq data here: single-cell data and the accompanying paper Leigh et. al 2018
Also, processed data can be found here and raw data here. For help with processing these data please see this GitHub page.
Other important resources: BLAST against the Pleurodeles waltl transcriptome on iNewt
Transcriptome and enrichment data from Bryant et al 2017. Genome resources, BLAST, datasets and other tools at Sal-site and axolotl-omics